Convergent extension during gastrulation in zebrafish

2P microscopy movie of anterior axial mesendoderm progenitor (prechordal plate progenitor) cell ingression at the onset of gastrulation – cells are stained with cytoplasmic and plasma membrane-anchored GFP. Movie courtesy Carl-Philipp Heisenberg.

 

Yolk syncytial layer formation in zebrafish

2P microscopy movie of yolk syncytial layer (YSL) formation at the 128-cell stage - cells are stained with cytoplasmic and plasma membrane-anchored GFP. Movie courtesy Carl-Philipp Heisenberg.

 

Kupffer’s vesicle formation in zebrafish

2P microscopy movie of Kupffer’s vesicle formation at the end of gastrulation - cells are stained with cytoplasmic and plasma membrane-anchored GFP. Movie courtesy Carl-Philipp Heisenberg.

 

Cell movements at the onset of gastrulation in zebrafish

2P microscopy movie of dorsal forerunner cell movements at the onset of gastrulation - cells are stained with cytoplasmic and plasma membrane-anchored GFP. Movie courtesy Carl-Philipp Heisenberg.

 

Paraxial mesendoderm progenitor cell ingression at the onset of gastrulation in zebrafish

2P microscopy movie of paraxial mesendoderm progenitor cell ingression at the onset of gastrulation - cells are stained with cytoplasmic and plasma membrane-anchored GFP (one exemplary ingressing mesendoderm progenitor cell is marked in red and one non-ingressing ectoderm progenitor cell is marked in green). Movie courtesy Carl-Philipp Heisenberg.

 

Yolk syncytial layer nuclei migration during gastrulation in zebrafish

2P microscopy movie of yolk syncytial layer (YSL) nuclei migration during gastrulation – the nuclei are marked by Histone-GFP and tracked with a custom-built cell/nuclei tracking software. Movie courtesy Carl-Philipp Heisenberg.