Web Topic 12.7 Proteins and RNAs: Signaling Molecules in the Phloem

Susan Dunford, University of Cincinnati, Cincinnati, OH, USA

Revised by Alexander Schulz, University of Copenhagen, Copenhagen, Denmark
(September 2021)

Proteins as Signal Molecules

Proteins synthesized in companion cells can clearly enter the sieve elements through the plasmodesmata that connect the two cell types. As noted in textbook chapter 12, the P-proteins in cucurbit exudate, PP1 and PP2, are synthesized in companion cells. The sieve-pore-plasmodesmata contacts connecting companion cells and sieve elements must thus allow these macromolecules to move across them. Furthermore, subunits of PP1 and PP2 move with the translocation stream to sink tissues.

Subunits of P-proteins from pumpkin (Cucurbita maxima) can move across graft unions from a pumpkin stock (basal graft partner) to a cucumber (Cucumis sativus) scion (upper graft partner), as well as from a pumpkin scion to a cucumber stock. Both proteins can move from the sieve elements to companion cells; neither protein is able to move beyond the sieve element–companion cell complex (Golecki et al., 1999; la Cour Petersen et al., 2005). Furthermore, no definite signaling function has yet been established for either protein.

Other proteins move in the translocation stream from sources to sinks. For example, passive movement of proteins from companion cells to sieve elements has been demonstrated in Arabidopsis and tobacco plants. These plants were transformed with the gene for green fluorescent protein (GFP) from jellyfish, under control of the SUC2 promoter from Arabidopsis. The SUC2 sucrose–H+ symporter is synthesized within the companion cells, so proteins expressed under the control of its promoter, including GFP, are also synthesized in the companion cells. GFP, which is localized by its fluorescence after excitation with blue light, moves through plasmodesmata from companion cells into sieve elements of source leaves (see textbook Figure 12.24B) and migrates within the phloem to sink tissues.

Finally, the jellyfish green fluorescent protein is unloaded symplasmically through the plasmodesmata into sink tissues, such as seed coats, anthers, root tips, and mesophyll cells in importing leaves (see textbook Figure 12.24C) (Imlau et al., 1999; Stadler et al., 2005). Because jellyfish GFP is unlikely to possess specific sequences for interaction with plasmodesmatal structures, its movement into and out of sieve elements is likely to occur by passive diffusion. Overexpression of gene constructs in companion cells of scions move all the way to the root protophloem unloading zone of the non-transgenic graft partner (Paultre et al., 2016).

Phloem transport of proteins that modify cellular functions has also been demonstrated (Web Table 12.12.A below). For example, FLOWERING LOCUS T (FT) protein appears to be a significant component of the floral stimulus that moves from leaf to apex in response to conditions inductive for flowering (see textbook Chapter 20; Endo et al., 2018). The PRms protein is a pathogenesis-related protein that is involved in the defense response of maize against fungal pathogens. PRms protein moves into and through the phloem, as shown by its presence in tobacco phloem exudate and its movement across graft unions. PRms can move across graft unions from a transgenic stock to a non-transgenic control (Bortolotti et al., 2005). Unloading of the protein has not yet been demonstrated, however, nor is its exact mechanism of action known.

Clearly, proteins can be transported from the companion cells in the source through the intervening sieve elements to sink companion cells. However, little evidence exists for a similar movement of proteins synthesized outside the companion cells. Other signals from outside the sieve element–companion cell complex may give rise to the production of mobile proteins in the companion cells.

WEB TABLE 12.12.A
Functions of Some Soluble Proteins Found in Sieve Element Sap

FUNCTION

EXAMPLES

Sugar metabolism

Sucrose synthase, glycolytic enzymes

Protein degradation

Ubiquitin

Redox regulation

Thioredoxin h

Protein phosphorylation

Protein kinases

Protein folding

Chaperones

Antioxidant defense

A suite of enzymes and radical scavengers

Protection of phloem proteins from degradation plus defense against pathogens and phloem-feeding insects

Protease inhibitors

RNA-binding

CmPP16, PP2, glycine-rich RNA-binding protein

Flowering signal

FLOWERING LOCUS T protein

(Source: Hayashi et al., 2000; Walz et al., 2004; Aki et al., 2008; Gaupels et al., 2008.)

RNAs as Signal Molecules

RNAs transported in the phloem consist of endogenous mRNAs, pathogenic RNAs, and small RNAs associated with gene silencing (see textbook Chapter 3). Most of these RNAs appear to travel in the phloem as complexes of RNA and protein (ribonucleoproteins [RNPs]) (Kehr and Kragler, 2018; Xia and Zhang, 2020). RNPs in the phloem resemble the complexes formed by viral RNAs with their movement proteins (MPs). Viral “movement proteins” interact directly with plasmodesmata to allow the passage of viral nucleic acids between cells.

The mechanism of cell-to-cell movement of viruses may differ from the mechanism of their import into the phloem and long-distance transport. While the role of MPs has been established in cell-to-cell movement through plasmodesmata, viral coat proteins may or may not be required for cell-to-cell movement. While viral coat proteins are widely required for viral translocation in the phloem, MPs may not be required (Kehr and Buhtz, 2008). In addition, endogenous phloem proteins could be involved in the phloem transport of viruses. For example, PP2, a phloem structural protein well known for its ability to seal off damaged sieve elements, is found in the phloem exudate of cucumber in a complex with viroid (pathogenic) RNA. Further, PP2 and the viroid RNA are simultaneously translocated from a cucumber stock to a pumpkin scion. Following transport, the viroid is capable of causing disease symptoms in the pumpkin scion (Gomez and Pallas, 2004).

Posttranscriptional gene silencing (see textbook Chapter 3) is one system that clearly demonstrates the role of RNAs in long-distance signaling in the phloem (Van Bel, 2003). RNA silencing of gene expression causes degradation of target mRNAs, for example, excess or foreign mRNA transcribed from transgenes or viral nucleic acid. When the transgenes are homologous to host genes, the host genes are also silenced. RNA silencing is triggered by the presence of double-stranded RNAs, induced by the virus or transgene. The double-stranded RNAs are processed by proteins called Dicer-like proteins to form small RNAs called short interfering RNAs (siRNAs). These siRNAs are incorporated into complexes that carry out the degradation of the target mRNAs. In this way, the gene is “silenced” (Eamens et al., 2008).

RNA silencing plays a role not only in defense processes against foreign mRNAs but also in developmental processes. Small RNAs called microRNAs (miRNAs) are formed similarly to siRNAs and likewise are incorporated into complexes that mediate degradation of mRNA (plants) or inhibit translation of mRNA (animals and insects) (Eamens et al., 2008; see Chapters 3, 15 and 23).

Small RNAs appear to meet all the requirements for signaling molecules: entry into sieve elements and transport in the phloem to sink tissues, where functional changes are triggered. Small RNAs shown to be authentic regulatory RNAs (siRNA and miRNA) are found in phloem sap, and their levels respond to growth conditions and viral infection. Furthermore, phloem transport of small RNAs has been demonstrated (Kehr and Kragler, 2018). An siRNA complementary to a viral coat-protein gene sequence was found in the sap of a wild-type cucumber scion that had been grafted onto a squash stock silenced for transgene coat-protein expression. Silencing occurred in the tissues of a scion from a non-silenced line that had been grafted onto a stock from a silenced squash line; that is, the levels of coat protein mRNA were reduced in the non-silenced line to the same low levels as found in the silenced line (Yoo et al., 2004).

Transmission of a silencing signal in the opposite direction, from a silenced scion to a non-silenced rootstock, has also been demonstrated in Nicotiana benthamiana, a close relative of tobacco native to Australia. Radioactive phosphate was utilized as a phloem tracer, and source-sink relationships were manipulated by removing source leaves from the rootstock. The transport of the silencing signal followed the pattern of source-to-sink phloem transport from the scion to the rootstock (Tournier et al., 2006).

Finally, the RNA transported in the phloem can cause visible changes in the sink. Changes in phenotype (appearance) due to the transport of RNA to a scion has been shown in a number of grafting experiments: the changes include chlorosis in tobacco due to posttranscriptional gene silencing and changes in leaf morphology in tomato due to transport of a mutant homeobox mRNA. In both cases, RNA movement occurred in an acropetal (toward the apex) direction, but movement in the phloem was not confirmed. However, mRNA for a regulator of gibberellic acid responses (called GAI) was localized to sieve elements and companion cells of pumpkin and was found in pumpkin phloem sap.

Transgenic tomato plants expressing a mutant version of the regulator gene were dwarf and dark green. The mRNA for the mutant regulator was localized to sieve elements, was able to be transported across graft unions into wild-type scions, and was unloaded into apical tissues. As a result, the mutant phenotype developed in new growth on the wild-type scion (Haywood et al., 2005). In a final example, a transcription factor which regulates tuber formation in potato accumulates in leaves and stolons in response to inductive short days and moves basipetally across a graft union from the upper graft partner (scion, overexpressing the transcription factor) to the stolon tips (wild-type stock), the site of tuber induction. Increased tuber production results (Banerjee et al., 2006).

Plasmodesmata Function in Signaling

The endogenous proteins commandeered by viruses to facilitate their specific movement to and through plasmodesmata are thought to carry out similar functions for endogenous macromolecules. Proteins have been identified in phloem exudate that bind RNA and increase the size exclusion limit of plasmodesmata between mesophyll cells (for example, PP2 and CmPP16); in addition, proteins have been identified in plasmodesmata that interact with the exudate proteins (for example, NtNCAPP1). The control over plasmodesmatal transport exerted by NtNCAP1 appears to be specific and selective (Ding et al., 2003).

It is believed that proteins such as CmPP16 are involved in macromolecular trafficking into sieve elements and long-distance transport in the phloem. CmPP16 from pumpkin was introduced into rice sieve elements through aphid stylets; the transport of CmPP16 to roots appeared to be selective (Aoki et al., 2005). Although it has not been shown to increase plasmodesmatal permeability, the maize pathogenesis-related protein (PRms) described earlier has been located in plasmodesmata between companion cells and sieve elements in transgenic tobacco plants. Localization was accomplished using an anti-PRms antibody. PRms increases allocation into sucrose, decreases allocation into starch, and thus increases sucrose efflux from source leaves of transgenic tobacco plants (Bortolotti et al., 2005).

For long-distance signaling, entry into sieve elements and exit from them is bound to the sieve-pore-plasmodesma contacts in leaves and along the transport phloem and to the specific funnel-shaped plasmodesmata found in root protophloem. The latter supplies carbohydrates and amino acids to root tip growth and thus fuels cell division and cellular expansion (Ross-Elliott et al., 2017). Unloading from protophloem sieve tubes into direct neighbors seems to be unspecific; however, companion cells in leaves and along the transport phloem need to be able to control the permeability of sieve pore-plasmodesma contacts and keep most of their cytosolic contents back. They cannot afford to lose all of it to the translocation stream. A filtering function was indeed shown for RNAs where only specific motifs allowed release into the sieve elements as seen by their transport across graft borders (Zhang et al., 2016). It is a matter of debate—and future research—whether e.g., ATP and other energy carriers are able freely to cross the interface between companion cell and sieve element or whether—and how—their movement between these cells is controlled.

References

Aki T, Shigyo M, Nakano R, Yoneyama T, Yanagisawa S (2008) Nano scale proteomics revealed the presence of regulatory proteins including three FT-Like proteins in phloem and xylem saps from rice. Plant Cell Physiol 49: 767–790.

Aoki K, Suzui N, Fujimaki S, Dohmae N, Yonekura-Sakakibara K, Fujiwara T, Hayashi H, Yamaya T, Sakakibara H (2005) Destination-selective long-distance movement of phloem proteins. Plant Cell 17: 1801–1814.

Banerjee AK, Chatterjee M, Yu Y, Suh SG, Miller WA, Hannapel DJ (2006) Dynamics of a mobile RNA of potato involved in a long-distance signaling pathway. Plant Cell 18: 3443–3457.

Bortolotti C, Murillo I, Fontanet P, Coca M, San Segundo B (2005) Long-distance transport of the maize pathogenesis-related PRms protein through the phloem in transgenic tobacco plants. Plant Science 168: 813–821.

Ding B, Itaya A, Qi Y (2003) Symplasmic protein and RNA traffic: regulatory points and regulatory factors. Curr Opin Plant Biol 6: 596–602.

Eamens A, Wang M-B, Smith NA, Waterhouse PM (2008) RNA Silencing in Plants: Yesterday, Today, and Tomorrow. Plant Physiology 147: 456–468.

Endo M, Yoshida M, Sasaki Y, Negishi K, Horikawa K, Daimon Y, Kurotani KI, Notaguchi M, Abe M, Araki T (2018) Re-Evaluation of Florigen Transport Kinetics with Separation of Functions by Mutations That Uncouple Flowering Initiation and Long-Distance Transport. Plant Cell Physiol 59: 1621–1629.

Gaupels F, Buhtz A, Knauer T, Deshmukh S, Waller F, van Bel AJ, Kogel KH, Kehr J (2008) Adaptation of aphid stylectomy for analyses of proteins and mRNAs in barley phloem sap. J Exp Bot 59: 3297–3306.

Golecki B, Schulz A, Thompson GA (1999) Translocation of structural P proteins in the phloem. Plant Cell 11: 127–140.

Gomez G, Pallas V (2004) A long-distance translocatable phloem protein from cucumber forms a ribonucleoprotein complex in vivo with Hop stunt viroid RNA. Journal of Virology 78: 10104–10110.

Hayashi H, Fukuda A, Suzui N, Fujimaki S (2000) Proteins in the sieve element-companion cell complexes: their detection, localization and possible functions. Australian Journal of Plant Physiology 27: 489–496.

Haywood V, Yu TS, Huang NC, Lucas WJ (2005) Phloem long-distance trafficking of Gibberellic acid-insensitive RNA regulates leaf development. Plant Journal 42: 49–68.

Imlau A, Truernit E, Sauer N (1999) Cell-to-cell and long-distance trafficking of the green fluorescent protein in the phloem and symplastic unloading of the protein into sink tissues. Plant Cell 11: 309–322.

Kehr J, Buhtz A (2008) Long distance transport and movement of RNA through the phloem. J Exp Bot 59: 85–92.

Kehr J, Kragler F (2018) Long distance RNA movement. New Phytologist 218: 29–40.

la Cour Petersen M, Hejgaard J, Thompson GA, Schulz A (2005) Cucurbit phloem serpins are graft-transmissible and appear to be resistant to turnover in the sieve element-companion cell complex. J Exp Bot 56: 3111–3120.

Paultre DSG, Gustin MP, Molnar A, Oparka KJ (2016) Lost in Transit: Long-Distance Trafficking and Phloem Unloading of Protein Signals in Arabidopsis Homografts. Plant Cell 28: 2016–2025.

Ross-Elliott TJ, Jensen KH, Haaning KS, Wagner BM, Knoblauch J, Howell AH, Mullendore DL, Monteith AG, Paultre D, Yan DW, Otero S, Bourdon M, Sager R, Lee JY, Helariutta Y, Knoblauch M, Oparka KJ (2017) Phloem unloading in Arabidopsis roots is convective and regulated by the phloem pole pericycle. Elife 6.

Stadler R, Wright KM, Lauterbach C, Amon G, Gahrtz M, Feuerstein A, Oparka KJ, Sauer N (2005) Expression of GFP-fusions in Arabidopsis companion cells reveals non-specific protein trafficking into sieve elements and identifies a novel post-phloem domain in roots. Plant Journal 41: 319–331.

Tournier B, Tabler M, Kalantidis K (2006) Phloem flow strongly influences the systemic spread of silencing in GFP Nicotiana benthamiana plants. Plant Journal 47: 383–394.

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Walz C, Giavalisco P, Schad M, Juenger M, Klose J, Kehr J (2004) Proteomics of curcurbit phloem exudate reveals a network of defence proteins. Phytochemistry 65: 1795–1804.

Xia C, Zhang C (2020) Long-Distance Movement of mRNAs in Plants. Plants 9: 731.

Yoo BC, Kragler F, Varkonyi-Gasic E, Haywood V, Archer-Evans S, Lee YM, Lough TJ, Lucas WJ (2004) A systemic small RNA signaling system in plants. Plant Cell 16: 1979–2000.

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