Chapter 8 Multiple Choice Questions

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. What type of mass spectrometer is used for radiocarbon dating?

Accelerator Mass Spectrometer correct incorrect

Quadrupole Mass Spectrometer correct incorrect

Sector Mass Spectrometer correct incorrect

Hybrid Q-TOF Mass Spectrometer correct incorrect

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. In biotransformation studies what type of mass spectrometer is typically used for neutral loss scanning in order to determine the metabolised form of a drug?

Magnetic sector correct incorrect

Triple quadrupole correct incorrect

Q-TOF correct incorrect

Ion Trap correct incorrect

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. What has been one of the most successful areas of clinical application for mass spectrometry since the 1990s?

Anti-doping correct incorrect

Proteomics correct incorrect

Metabolomics correct incorrect

Inborn errors of metabolism correct incorrect

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. Which of the following is currently a significant challenge in metabolomics

Comprehensive identification of metabolites correct incorrect

Accurate mass measurements correct incorrect

Chromatographic peaks shape correct incorrect

High resolution measurements correct incorrect

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. What is one way in which mass spectrometry plays an important role in precision medicine?

Mass spectrometers can monitor an individual's health in real time correct incorrect

Systems biology associated with the proteome and metabolome of an individual may be useful to predicting susceptibility to disease and response to treatment correct incorrect

Most clinicians are now experts in mass spectrometry correct incorrect

The increased accuracy of mass spectrometers has now made them suitable for diagnosing disease routinely. correct incorrect

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. Which of the following best describes bottom-up proteomics?

Analysing intact proteins by MS and MS/MS correct incorrect

Converting proteins to peptides and analysing by MS and MS/MS correct incorrect

Purifying proteins correct incorrect

Focussing on the analysis of small proteins only correct incorrect

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. Which of the following is a recognised method in quantitative proteomics?

iQUAN correct incorrect

iCAL correct incorrect

iEYE correct incorrect

iTRAQ correct incorrect

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. The neutral loss of 98 Da from a peptide ion is diagnostic of which common protein post-translational modification?

Glycosylation correct incorrect

Phosphorylation correct incorrect

Oxidation correct incorrect

Deamidation correct incorrect

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. Under denaturing electrospray ionization conditions a protein gives a spectrum with a base peak [M+30H]³⁰⁺ = m/z 917.67. Under native ESI conditions, the base peak is seen at [M+11H]¹¹⁺ = m/z 2547.1. What can be

The protein has a covalent modification of mass 307 Da correct incorrect

A ligand of mass 307 Da is non-covalently bound to the protein correct incorrect

The protein has a covalent modification of mass 507 Da correct incorrect

A ligand of mass 507 Da is non-covalently bound to the protein correct incorrect

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. Which of following ionization methods is not currently used in mass spectrometry imaging?

EI correct incorrect

MALDI correct incorrect

SIMS correct incorrect

DESI correct incorrect