Chapter 3 Multiple choice questions

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. Which of the following best describes what can be done by variations on the CRISPR/Cas9 DNA editing system?

Targeted destruction of a gene correct incorrect

Targeted alteration of base sequences at specific sites in the genome correct incorrect

Targeted insertion of a sequence into a gene correct incorrect

All of the above correct incorrect

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. In the Sc2.0 yeast re-engineering project, why are all of the TAG stop codons being replaced by TGA (and existing TGA left as they are)?

To test whether the TAG sequence is necessary for the yeast to live correct incorrect

Because the TGA sequence works better as a stop codon correct incorrect

To allow a new TAG-recognizing tRNA to be introduced, to build a 21st amino acid into proteins correct incorrect

To economize on the number of tRNA genes that have to be added to the neochromosome 17 correct incorrect

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. Which of the following best defines a 'safe harbour site' in the genome?

A site where a construct will not interfere with the normal functions of a host cell correct incorrect

A site where a construct will not be silenced by chromatin modification correct incorrect

A site free from recombination correct incorrect

A site where integration is easy correct incorrect

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. Which of the following represents the engineering cycle?

Design-build-test correct incorrect

Build-test-design correct incorrect

Design-test-build correct incorrect

Build-test-build correct incorrect

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. Which one of the following correctly describes the action of restriction enzymes (= restriction endonucleases)?

The enzyme restricts the length of DNA. correct incorrect

The enzyme cuts DNA at a particular sequence. correct incorrect

The enzyme restricts the site into which DNA can integrate. correct incorrect

The enzyme restricts the ability of the cell to alter chromatin. correct incorrect

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. Which of the following is NOT a recognized method to transfect cells with engineered DNA?

Electroporation correct incorrect

Microinjection correct incorrect

Lyophilization correct incorrect

Lipofection correct incorrect

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. Which of the following best describes homologous recombination?

Recombination between similar cells correct incorrect

Recombination between lengths of DNA with identical sequences correct incorrect

Recombination between Cas9 and gRNA correct incorrect

Recombination between different genes coding for similar functions correct incorrect

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. Which of the following best defines phenotyping of a cell?

Establishing the sequence of a gene correct incorrect

Measuring phenylalanine content of a cell correct incorrect

Establishing phenol tolerance of a cell correct incorrect

Establishing the behaviour / structure / contents of a cell correct incorrect

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. Which of the following contain proteins that use metal ions?

ZFNs correct incorrect

CRISPR effectors correct incorrect

Restriction endonucleases correct incorrect

Lipofection reagents correct incorrect

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. Which of the following is NOT an aspect of the inflammatory response?

Heat correct incorrect

Redness correct incorrect

Swelling correct incorrect

Dehydration correct incorrect