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Monoclonal antibodies can be made by inoculating an animal (such as a mouse) with an antigen, providing the animal time to mount an immune response, collecting the B cells from the animal, and then fusing the B cells to long-lived myeloma cells—thereby creating hybridomas that can be tested for the production of useful antibodies.
Textbook Reference: Detection of Nucleic Acids and Proteins, p. 141