Chapter 5 Self-test questions

. Which of the following statements about column chromatography is correct?

Affinity chromatography involves the attachment to the column matrix of ionic groups known that bind to the wanted protein.

In reverse phase chromatography the wanted protein can be selectively eluted by solutions of different hydrophobicities or ionic strengths.

Ion-exchange chromatography separates proteins according to their size.

Gel-filtration chromatography separates proteins on their ability to bind to specific groups on the column matrix.

. Which of the following statements about SDS polyacrylamide gel electrophoresis is correct?

Wanted proteins can be tested for their biological activity after separation by SDS polyacrylamide gel electrophoresis.

Proteins are solubilized but not denatured when separated by SDS polyacrylamide gel electrophoresis.

SDS polyacrylamide gel electrophoresis separates proteins on the basis of charge.

SDS polyacrylamide gel electrophoresis separates proteins on the basis of size.

. Which of the following statements about isoelectric focusing is correct?

Proteins separated by isoelectric focusing cannot be tested for biological activity.

Proteins separated by isoelectric focusing can be tested for biological activity.

The separation of proteins by isoelectric focusing is only based on charge.

The separation of proteins by isoelectric focusing is only based on size.

. Which of the following statements about the use of mass spectrometry in protein investigation is correct?

Mass spectrometry involves ionized molecules in the liquid phase.

Mass spectrometry is used for analysing the three-dimensional shape of peptides.

Ionic fragments are separated according to their mass-to-charge ratio in mass spectrometry.

Mass spectrometry involves the separation of ionic fragments on a gel.

. Which of the following statements about Western Blotting is correct?

The detection of a particular protein by western blotting relies on the very specific interaction between the protein and its antibody.

The detection of a particular protein by western blotting relies on labelling the protein with a specific dye.

The detection of a particular protein by western blotting relies on labelling the antibody with a specific dye.

The detection of a particular protein by western blotting relies on the denaturation of the protein.

. Which of the following statements about Nuclear Magnetic Resonance (NMR) is correct?

A disadvantage of NMR analysis is that it requires a large amount of protein.

NMR is used to determine the amino acid sequence of proteins.

NMR requires crystallisation of proteins.

The majority of known protein structures have been determined using NMR.

. Which of the following statements about protein separation by gel filtration are correct? Please select all that apply.

When a mixture of proteins is separated by gel filtration, the smallest molecular weight protein is eluted first.

When a mixture of proteins is separated by gel filtration, the largest molecular weight protein is eluted first.

When a mixture of proteins is separated by gel filtration, the smallest molecular weight protein is eluted last.

When a mixture of proteins is separated by gel filtration, the smallest molecular weight proteins flow around the beads.

. Which of the following statements about the use of mass spectrometry in protein investigation are correct? Please select all that apply.

Mass spectrometry is used to rapidly determine the molecular weight of a protein.

Mass spectrometry is only used to determine the sequence of peptides.

Mass spectrometry can be used to investigate post-translational modifications.

Mass spectrometry involves separating ionic fragments on a gel.

. Which of the following statements about two-dimensional gel electrophoresis are correct? Please select all that apply.

SDS gel electrophoresis and isoelectric focusing both separate native proteins. correct incorrect

SDS gel electrophoresis and isoelectric focusing both make use of the migration of proteins in an electric field. correct incorrect

SDS gel electrophoresis and isoelectric focusing both require a pH gradient. correct incorrect

SDS gel electrophoresis and isoelectric focusing together make up the process of two-dimensional gel electrophoresis. correct incorrect

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. Which of the following statements about the determination of the three-dimensional structure of proteins are correct? Please select all that apply.

X-ray diffraction requires that the protein first be crystallized.

X-ray diffraction requires that the protein first be solubilized.

X-ray diffraction records electron densities around the atoms of a protein.

X-ray diffraction is performed to separate proteins.

. Which of the following techniques used to study proteins has been most useful in opening up the field of proteomics?

Affinity chromatography.

Mass spectrometry.

Nuclear magnetic resonance spectroscopy.

X-ray diffraction.

. When information can be gained from 'homology modelling' of two proteins with similar amino acid sequences?

The amino acid sequence of one protein if that of the other is known.

The isoelectric point of one protein if that of the other is known.

An estimate of the 3-D structure of one protein if the structure of the other is known.

An estimate of their 3-D structures even if neither structure is known.

Chapter 5 Self-test questions

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