Chapter 24 Self-test questions

. Which of the following is true of RNA synthesis (transcription)?

RNA synthesis is always in the 5' – 3' direction.

RNA polymerase needs a primer to initiate transcription.

In transcription, U is inserted opposite T.

New nucleotides are added on to the 2' OH of the ribose sugar.

. In bacterial promoters, which of the following describes the 'Pribnow box'?

The 5' untranslated region.

The –10 box.

The –35 box.

The termination sequence.

. The role of the sigma factor in bacterial RNA polymerase is:

to catalyse RNA synthesis.

to position RNA polymerase correctly on the template DNA.

to terminate RNA synthesis.

to unwind the DNA template.

. Which of the following statements regarding termination of transcription in prokaryotes is correct?

In Rho dependent termination the Rho factor moves along the DNA template ahead of the RNA polymerase.

Rho factor has topoisomerase activity for relieving supercoiling.

Termination often involves a stem-loop structure forming in the RNA transcript.

Termination often involves a stem-loop structure forming in the DNA template.

. What is the role of eukaryotic RNA polymerase I?

Transcription of mRNA only.

Transcription of mRNA, rRNA, and tRNA.

Transcription of 'small' RNAs including tRNAs, 5S RNAs, and snRNAs.

Transcription of the major rRNA transcript.

. Which of the following does the abbreviation TBP stand for?

TATA-box binding protein.

Transcription associated factor.

Transcription factor binding protein.

TATA box polymerase.

. Which of the following best describes the 'cap' modification of eukaryotic mRNA?

A modified guanine nucleotide added to the 3' end of the transcript.

A modified guanine nucleotide added to the 5' end of the transcript.

A string of adenine nucleotides added to the 3' end of the transcript.

A string of adenine nucleotides added to the 5' end of the transcript.

. Which of the following statements regarding splicing of eukaryotic mRNA transcripts is correct?

Exons are spliced out and introns are retained in the mature mRNA transcript.

Several reactions in the splicing process involve hydrolysis of ATP.

Small nuclear RNAs are retained in the mature mRNA transcript.

Splicing takes place in the cytosol.

. Which of the following statements about mRNA splicing is true?

The existence of split genes has no advantage

Self-splicing introns do not require the help of any protein for splicing to occur accurately.

β-thalassaemia results from a genetic defect in the spliceosome.

Splicing occurs in the cytosol.

. Is the following statement true or false? 'Eukaryotic mRNA is transcribed by RNA polymerase II.'

True

False

. The polyA tail on eukaryotic mRNA is encoded by a long string of Ts at the end of the gene. Is this statement true or false?

True.

False.

. Which of the following occur in both eukaryotic and bacterial transcription? Please select all that apply.

5' cap.

polyA tail.

Promoter.

DNA-dependent RNA polymerase.

Chapter 24 Self-test questions

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