Alvarez, I. S. and G. C. Schoenwolf. 1992. Expansion of surface epithelium provides the major extrinsic force for bending of the neural plate. J. Exp. Zool. 261: 340–348.
PubMed Link

Anderson, D. D., C. F. Woeller, E. P. Chiang, B. Shane and P. J. Stover. 2012. Serine hydroxymethyltransferase anchors de novo thymidylate synthesis pathway to nuclear lamina for DNA synthesis. J. Biol. Chem. 287: 7051–7062.
PubMed Link

Archer, T. C., J. Jin and E. S. Casey. 2011. Interaction of Sox1, Sox2, Sox3 and Oct4 during primary neurogenesis. Dev. Biol. 350: 429–40.
PubMed Link

Artama, M., A. Auvinen, T. Raudaskoski, I. Isojärvi, and J. Isojärvi. 2005. Antiepileptic drug use of women with epilepsy and congenital malformations in offspring. Neurology 64: 1874–1878.
PubMed Link

Balinsky, B. I. 1981. Introduction to Embryology, 5th Ed. Saunders, Philadelphia, PA.

Bassuk, A. G. and Z. Kibar. 2009. Genetic basis of neural tube defects. Semin. Pediatr. Neurol. 16: 101–110.
PubMed Link

Briscoe, J., L. Sussel, P. Serup, D. Hartigan-O’Connor, T. M. Jessell, J. L. R. Rubenstein and J. Ericson. 1999. Homeobox gene Nkx2.2 and specification of neuronal identity by graded Sonic hedgehog signaling. Nature 398: 622–627.
PubMed Link

Bu, P., Y. A. Evrard, G. Lozano, and S. Y. R. Dent. 2007. Loss of Gcn5 acetyltransferase activity leads to neural 
tube closure defects and exencephaly in mouse embryos. Mol. Cell. Biol. 27: 3405–3416.
PubMed Link

Cambray, N. and V. Wilson. 2007. Two distinct sources for a population of maturing axial progenitors. Development 134: 2829–2840.
PubMed Link

Catala, M., M.-A. Teillet and N. M. Le Douarin. 1995. Organization and development of the tailbud analyzed with the quail-chick chimaera system. Mech. Dev. 51: 51–65.
PubMed Link

Catala, M., M.-A. Teillet, E. M. De Robertis and N. M. Le Douarin. 1996. A spinal cord fate map in the avian embryo: While regressing, Hensen’s node lays down the notochord and floor plate thus joining the spinal cord lateral walls. Development 122: 2599–2610.
PubMed Link

Catela, C., M. M. Shin, and J. S. Dasen. 2015. Assembly and function of spinal circuits for motor control. Annu. Rev. Cell Dev. Biol. 31: 669–698.
PubMed Link

CDC (Centers for Disease Control). 1992. Recommendations for the use of folic acid to reduce the number of cases of spina bifida and other neural tube defects. Morb. Mortal. Wkly. Rep. 41: 1–7.
PubMed Link

Chapman, D. L. and V. E. Papaioannou. 1998. Three neural tubes in mouse embryos with mutations in the T-box gene Tbx6. Nature 391: 695–697.
PubMed Link

Cohen, M., A. Kicheva, A. Ribeiro, R. Blassberg, K. M. Page, C. P. Barnes, and J. Briscoe. 2015. Ptch1 and Gli regulate Shh signalling dynamics via multiple mechanisms. Nat. Commun. 6: 6709.
PubMed Link

Copp, A. J., N. D. E. Greene and J. N. Murdoch. 2003. The genetic basis of mammalian neurulation. Nat. Rev. Genet. 4: 784–93.
PubMed Link

Czeizel, A. E. and I. Dudás. 1992. Prevention of first occurrence of neural tube defects by periconceptional vitamin supplementation. N. Engl. J. Med. 327: 1832–1835.
PubMed Link

Dady, A., E. Havis, V. Escriou, M. Catala, and J. L. Duband. 2014. Junctional neurulation: a unique developmental program shaping a discrete region of the spinal cord highly susceptible to neural tube defects. J. Neurosci. 34: 13208–13221.
PubMed Link

De Crescenzo, H. and 9 others. 2021. Deficient or excess folic acid supply during pregnancy alter cortical neurodevelopment in mouse offspring. Cereb. Cortex 31: 635–649.
PubMed Link

Dessaud, E., L. and 8 others. 2007. Interpretation of the Sonic hedgehog morphogen gradient by a temporal adaptation mechanism. Nature 450: 717–720.
PubMed Link

Detrick, R. J., D. Dickey and C. R. Kintner. 1990. The effects of N-cadherin misexpression on morphogenesis in Xenopus embryos. Neuron 4: 493–506.
PubMed Link

Echelard, Y., D. J. Epstein, B. St.-Jacques, L. Shen, J. Mohler, J. A. McMahon and A. McMahon. 1993. Sonic hedgehog, a member of a family of putative signaling molecules, is implicated in the regulation of CNS polarity. Cell 75: 1417–1430.
PubMed Link

Feil, R., R. A. Waterland, M. F. Fraga, and R. L. Jirtle. 2012. Epigenetics and the environment: Emerging patterns and implications. Nat. Rev. Genet. 13: 97–109.
PubMed Link

Fournier-Thibault, C., C. Blavet, A. Jarov, F. Bajanca, S. Thorsteinsdóttir and J. L. Duband. 2009. Sonic hedgehog regulates integrin activity, cadherin contacts, and cell polarity to orchestrate neural tube morphogenesis. J. Neurosci. 29: 12506–12520.
PubMed Link

Fujimori, T., S. Miyatani and M. Takeichi. 1990. Ectopic expression of N-cadherin perturbs histogenesis in Xenopus embryos. Development 110: 97–104.
PubMed Link

Gallera, J. 1971. Primary induction in birds. Adv. Morphogenet. 9: 149–180.
PubMed Link

Gont, L. K., H. Steinbeisser, B. Blumberg and E. M. De Robertis. 1993. Tail formation as a continuation of gastrulation: The multiple cell populations of the Xenopus tailbud derive from the late blastopore lip. Development 119: 991–1004.
PubMed Link

Gouti, M., V. Metzis, and J. Briscoe. 2015. The route to spinal cord cell types: A tale of signals and switches. Trends Genet. 31: 282–289.
PubMed Link

Guthrie, S. and A. Lumsden. 1991. Formation and regeneration of rhombomere boundaries in the developing chick hindbrain. Development 112: 221–229.
PubMed Link

Handrigan, G. R. 2003. Concordia discors: Duality in the origin of the vertebrate tail. J. Anat. 202: 255–67.
PubMed Link

Harland, R. 2000. Neural induction. Curr. Opin. Genet. Dev. 10: 357–362.
PubMed Link

Harrington, M. J., E. Hong and R. Brewster. 2009. Comparative analysis of neurulation: First impressions do not count. Mol. Reprod. Dev. 76: 954–965.
PubMed Link

Harris, M. J. and D. M. Juriloff. 2010. An update to the list of mouse mutants with neural tube closure defects and advances toward a complete genetic perspective of neural tube closure. Birth Defects Res. A: Clin. Mol. Teratol. 88: 653–669.
PubMed Link

Hashimoto, H., F. B. Robin, K. M. Sherrard, and E. M. Munro. 2015. Sequential contraction and exchange of apical junctions drives zippering and neural tube closure in a simple chordate. Dev. Cell. 32: 241–255.
PubMed Link

Henrique, D., E. Abranches, L. Verrier and K. G. Storey. 2015. Neuromesodermal progenitors and the making of the spinal cord. Development 142: 2864–2875.
PubMed Link

Jacobson, A. G. and J. G. Moury. 1995. Tissue boundaries and cell behavior during neurulation. Dev. Biol. 171: 98–110.
PubMed Link

Jessell, T. M. 2000. Neuronal specification in the spinal cord: Inductive signals and transcriptional codes. Nat. Rev. Genet. 1: 20–29.
PubMed Link

Kancherla, V., K. Wagh, P. Priyadarshini, H. Pachón and G. P. Oakley Jr. 2022. A global update on the status of prevention of folic acid-preventable spina bifida and anencephaly in year 2020. Birth Defects Res. 114: 1392–1403.
PubMed Link

Lawson, A., H. Anderson and G. C. Schoenwolf. 2001. Cellular mechanisms of neural fold formation and morphogenesis in the chick embryo. Anat. Rec. 262: 153–168.
PubMed Link

Lee, S.-K. and S. L. Pfaff. 2001. Transcriptional networks regulating neuronal identity in the developing spinal cord. Nat. Neurosci. Suppl. 4: 1183–1191.
PubMed Link

Lele, Z. and 7 others. 2002. Parachute/N-cadherin is required for morphogenesis and maintained integrity of the zebrafish neural tube. Development 129: 3281–3294.
PubMed Link

Li, B., L. Brusman, J. Dahlka and L. A. Niswander LA. 2022. TMEM132A ensures mouse caudal neural tube closure and regulates integrin-based mesodermal migration. Development 149: dev200442.
PubMed Link

Li, H., J. Zhang and L. Niswander. 2018. Zinc deficiency causes neural tube defects through attenuation of p53 ubiquitylation. Development 145: dev169797.
PubMed Link

Liem, K. F., Jr., G. Tremml and T. M. Jessell. 1997. A role for the roof plate and its resident TGF-beta-related proteins in neuronal patterning in the dorsal spinal cord. Cell 91: 127–138.
PubMed Link

Liem, K. F., Jr., G. Tremml, H. Roelink and T. M. Jessell. 1995. Dorsal differentiation of neural plate cells induced by BMP-mediated signals from epidermal ectoderm. Cell 82: 969–979.
PubMed Link

Liem, K. F., Jr., T. M. Jessell and J. Briscoe. 2000. Regulation of the neural patterning activity of Sonic hedgehog by secreted BMP inhibitors expressed by notochord and somites. Development 127: 4855–4866.
PubMed Link

Lowery, L. A. and H. Sive. 2004. Strategies of neurulation and a re-evaluation of teleost neural tube formation. Mech. Dev. 121: 1189–1197.
PubMed Link

Lumsden, A. 2004. Segmentation and compartition in the early avian hindbrain. Mech. Dev. 121: 1081–1088.
PubMed Link

Lumsden, A. and R. Keynes. 1989. Segmental patterns of neuronal development in the chick hindbrain. Nature 337: 424–428.
PubMed Link

Marean, A., A. Graf, Y. Zhang and L. Niswander. 2011. Folic acid supplementation can adversely affect murine neural tube closure and embryonic survival. Hum. Molec. Genet. 20: 3678–3683.
PubMed Link

Massarwa, R. and L. Niswander. 2013. In toto live imaging of mouse morphogenesis and new insights into neural tube closure. Development 140: 226–236.
PubMed Link

McShane, S. G., M. A. Molè, D. Savery, N. D. Greene, P. P. Tam, and A. J. Copp. 2015. Cellular basis of neuroepithelial bending during mouse spinal neural tube closure. Dev. Biol. 404: 113–124.
PubMed Link

Milunsky, A., H. Jick, S. S. Jick, C. L. Bruell, D. S. Maclaughlen, K. J. Rothman and W. Willett. 1989. Multivitamin folic acid supplementation in early pregnancy reduces the prevalence of neural tube defects. J. Am. Med. Assoc. 262: 2847–2852.
PubMed Link

Moore, K. L. and T. V. N. Persaud. 1993. Before We Are Born: Essentials of Embryology and Birth Defects. W. B. Saunders, Philadelphia, PA.

Moury, J. D. and G. C. Schoenwolf. 1995. Cooperative model of epithelial shaping and bending during avian neurulation: Autonomous movements of the neural plate, autonomous movements of the epidermis, and interactions in the neural plate/epidermis transition zone. Dev. Dyn. 204: 323–337.
PubMed Link

Muhr, J., E. Andersson, M. Persson, T. M. Jessell and J. Ericson. 2001. Groucho-mediated transcriptional repression establishes progenitor cell pattern and neuronal fate in the ventral spinal cord. Cell 104: 861–873.
PubMed Link

Murko, C., S. Lagger, M. Steiner, C. Seiser, C. Schoefer, and O. Pusch. 2013. Histone deacetylase inhibitor trichostatin A induces neural tube defects and promotes neural crest specification in the chicken neural tube. Differentiation 85: 55–66.
PubMed Link

Nakatsu, T., C. Uwabe and K. Shiota. 2000. Neural tube closure in humans initiates at multiple sites: Evidence from human embryos and implications for the pathogenesis of neural tube defects. Anat. Embryol. 201: 455–466.
PubMed Link

Nguyen, V. H., J. Trout, S. A. Connors, P. Andermann, E. Weinberg and M. C. Mullins. 2000. Dorsal and intermediate neuronal cell types of the spinal cord are established by a BMP signaling pathway. Development 127:1209–1220.
PubMed Link

Nievelstein, R. A. J., N. G. Hartwig, C. Vermeij-Keers and J. Valk. 1993. Embryonic development of the mammalian caudal neural tube. Teratology 48: 21–31.
PubMed Link

Niwa, M. and 10 others. 2013. Adolescent stress-induced epigenetic control of dopaminergic neurons via glucocorticoids. Science 339: 335–339.
PubMed Link

O’Rahilly, R. and F. Müller. 2002. The two sites of fusion of the neural folds and the two neuropores of the human embryo. Teratology 65: 162–170.
PubMed Link

Pasteels, J. 1937. Etudes sur la gastrulation des vertébrés méroblastiques. III. Oiseaux. IV. Conclusions générales. Arch. Biol. 48: 381–488.

Patten, B. M. 1971. Early Embryology of the Chick, 5th Ed. McGraw-Hill, New York.

Pei, P. and 7 others. 2019. Folate deficiency induced H2A ubiquitination to lead to downregulated expression of genes involved in neural tube defects. Epigenetics Chromatin 12: 69.
PubMed Link

Placzek, M., M. Tessier-Lavigne, T. Yamada, T. Jessell and J. Dodd. 1990. Mesodermal control of neural cell identity: Floor plate induction by the notochord. Science 250: 985–988.
PubMed Link

Pyrgaki, C., A. Liu, and L. Niswander. 2011. Grainyhead-like 2 regulates neural tube closure and adhesion molecule expression during neural fold fusion. Dev. Biol. 353: 38–49.
PubMed Link

Pyrgaki, C., P. Trainor, A. K. Hadjantonakis and L. Niswander. 2010. Dynamic imaging of mammalian neural tube closure. Dev. Biol. 344: 941–947.
PubMed Link

Rifat, Y. and 7 others. 2010. Regional neural tube closure defined by the Grainy head-like transcription factors. Dev. Biol. 345: 237–245.
PubMed Link

Roelink, H., J. A. Porter, C. Chiang, Y. Tanabe, D. T. Chang, P. A. Beachy and T. M. Jessell. 1995. Floor plate and motor neuron induction by different concentrations of amino-terminal cleavage product of Sonic hedgehog autoproteolysis. Cell 81: 445–455.
PubMed Link

Sasai, N., E. Kutejova, and J. Briscoe. 2014. Integration of signals along orthogonal axes of the vertebrate neural tube controls progenitor competence and increases cell diversity. PLOS Biol. 12: e1001907.
PubMed Link

Sausedo, R. A., J. L. Smith and G. C. Schoenwolf. 1997. Role of oriented cell division in shaping and bending of the neural plate. J. Comp. Neurol. 381: 473–488.
PubMed Link

Schoenwolf, G. C. 1984. Histological and ultrastructural studies of secondary neurulation in mouse embryos. Am. J. Anat. 169: 361–376.
PubMed Link

Schoenwolf, G. C. 1991a. Cell movements driving neurulation in avian embryos. Development 2: 157–168.
PubMed Link

Schoenwolf, G. C. 1991b. Cell movements in the epiblast during gastrulation and neurulation in avian embryos. In R. Keller et al. (eds)., Gastrulation. Plenum, New York, 1–28.

Schoenwolf, G. C. and J. Delongo. 1980. Ultrastructure of secondary neurulation in the chick embryo. Am. J. Anat. 158: 43–63.
PubMed Link

Schoenwolf, G. C. and J. L. Smith. 1990. Epithelial cell wedging: A fundamental cell behavior contributing to hinge point formation during epithelial morphogenesis. In R. E. Keller and D. Fristrom (eds.). Control of Morphogenesis, Vol. 1. Saunders, London, 325–334.

Schoenwolf, G. C. and M. V. Franks. 1984. Quantitative analyses of changes in cell shapes during bending of the avian neural plate. Dev. Biol. 105: 257–272.
PubMed Link

Shimokita, E., I. Kashin and Y. Takahashi. 2010. Secondary neurulation: A novel model to study tubulogenesis. Poster presentation.

Shpargel, K. B., T. Sengoku, S. Yokoyama, and T. Magnuson. 2012. UTX and UTY demonstrate histone demethylase-independent function in mouse embryonic development. PLOS Genet. 8: e1002964.
PubMed Link

Shyamasundar, S., S. P. Jadhav, B. H. Bay, S. S. W. Tay, S. D. Kumar, D. Rangasamy, and S. T. Thameem Dheen. 2013. Analysis of epigenetic factors 
in mouse embryonic neural stem cells exposed to hyperglycemia. PLOS ONE 8: e65945.
PubMed Link

Smith, J. L. and G. C. Schoenwolf. 1987. Cell cycle and neuroepithelial cell shape during bending of the chick neural plate. Anat. Rec. 218: 196–206.
PubMed Link

Smith, J. L. and G. C. Schoenwolf. 1988. Role of cell cycle in regulating neuroepithelial cell shape during bending of the chick neural plate. Cell Tissue Res. 252: 491–500.
PubMed Link

Smith, J. L. and G. C. Schoenwolf. 1991. Further evidence of extrinsic forces in bending of the neural plate. J. Comp. Neurol. 307: 225–236.
PubMed Link

Smith, J. L. and G. C. Schoenwolf. 1997. Neurulation: Coming to closure. Trends Neurosci. 20: 510–517.
PubMed Link

Stern, C. D. 2005. Neural induction: Old problem, new findings, yet more questions. Development 132: 2007–2021.
PubMed Link

Steventon, B. and A. Martinez Arias. 2017. Evo-engineering and the cellular and molecular origins of the vertebrate spinal cord. Dev. Biol. 432: 3–13.
PubMed Link

Takemoto, T., M. Uchikawa, M. Yoshida, D. M. Bell, R. Lovell-Badge, V. E. Papaioannou and H. Kondoh. 2011. Tbx6-dependent Sox2 regulation determines neural or mesodermal fate in axial stem cells. Nature 470: 394–398.
PubMed Link

Tye, K. M. and 12 others. 2013. Dopamine neurons modulate neural encoding and expression of depression-related behaviour. Nature 493: 537–541.
PubMed Link

Tzouanacou, E., A. Wegener, F. J. Wymeersch, V. Wilson and J. F. Nicolas. 2009. Defining the progression of lineage segregations during mammalian embryogenesis by clonal analysis. Dev. Cell 17: 365–376.
PubMed Link

Welstead, G. G., M. P. Creyghton, S. Bilodeau, A. W. Cheng, S. Markoulaki, R. A. Young, and R. Jaenisch. 2012. X-linked H3K27me3 demethylase Utx is required for embryonic development in a sex-specific manner. Proc. Natl. Acad. Sci. 109: 13004–13009.
PubMed Link

Werth, M. and 13 others. 2010. The transcription factor grainyhead-like 2 regulates the molecular composition of the epithelial apical junctional complex. Development 137: 3835–3845.
PubMed Link

Wilde, J. J., J. R. Petersen, and L. Niswander. 2014. Genetic, epigenetic, and environmental contributions to neural tube closure. Annu. Rev. Genet. 48: 583–611.
PubMed Link

Wilson, S. I. and T. Edlund. 2001. Neural induction: Toward a unifying mechanism. Nat. Neurosci. 4: 1161–1168.
PubMed Link

Wilson, V., I. Olivera-Martinez and K. G. Storey. 2009. Stem cells, signals and vertebrate body axis extension. Development 136: 1591–1604.
PubMed Link

Xiong, F. and 11 others. 2013. Specified neural progenitors sort to form sharp domains after noisy Shh signaling. Cell 153: 550-561.
PubMed Link

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