Further Development 15.1: Learn How These Four Pioneer Transcription Factors Differentiate Neural Crest Cell Fates

Neural Crest Cells and Axonal Specificity

When Foxd3, Snail, Sox9, and Sox10 are experimentally expressed in the lateral neural tube, the lateral neuroepithelial cells become neural crest-like, undergo epithelial-to-mesenchymal transition (EMT) and delaminate from the neuroepithelium:

  • Sox9 and Snail together are sufficient to induce EMT in neuroepithelial cells. Sox9 is also required for the survival of trunk neural crest cells after delamination (in the absence of Sox9, neural crest cells undergo apoptosis as soon as they delaminate).

  • Foxd3 may play many roles. It is needed for the expression of the cell surface proteins that are required for cell migration, and it also appears to be critical for the specification of ectodermal cells as neural crest. Inhibiting expression of the Foxd3 gene inhibits neural crest differentiation. Conversely, when Foxd3 is expressed ectopically by electroporating the active gene into neural plate cells, those neural plate cells express proteins characteristic of the neural crest (Nieto et al. 1994; Taneyhill et al. 2007; Teng et al. 2008).

  • The Sox10 gene appears to be one of the most critical regulators of neural crest specification. It is crucial not only for the delamination of neural crest cells from the neural tube, but also for the differentiation of the numerous neural crest lineages (Kelsh 2006; Betancur et al. 2010). Sox10 protein binds to the enhancers of numerous target genes that encode the neural crest effectors; these include the genes for some small G proteins, such as Rho GTPases, that allow cells to change shape and migrate; cell surface receptors, such as receptor tyrosine kinases and endothelin receptor (e.g., ENDRB2), that allow the neural crest cells to respond to patterning and chemotactic proteins in their environments; and transcription factors, such as MITF in the melanocyte lineage that forms pigment cells (see Figure 15.6; Simões-Costa and Bronner 2015).

Literature Cited

Betancur, P., M. Bronner-Fraser and T. Sauka-Spengler. 2010. Genomic code for Sox10 activation reveals a key regulatory enhancer for cranial neural crest.Proc. Natl. Acad. Sci. USA 107: 3570–3575.

PubMed Link

Kelsh, R. N. 2006. Sorting out Sox10 functions in neural crest development. Bioessays 28: 788–798.

PubMed Link

Nieto, M. A., M. G. Sargent, D. G. Wilkinson and J. Cooke. 1994. Control of cell behavior during vertebrate development by slug, a zinc finger gene. Science264: 835–839.

PubMed Link

Simões-Costa, M. and M. E. Bronner. 2015. Establishing neural crest identity: A gene regulatory recipe. Development 142: 242–257.

PubMed Link

Taneyhill, L. A., E. G. Coles and M. Bronner-Fraser. 2007. Snail2 directly represses cadherin6B during epithelial-to-mesenchymal transitions of the neural crest. Development 134: 1481–1490.

PubMed Link

Teng, L., N. A. Mundell, A. Y. Frist, Q. Wang and P. A. Labosky. 2008. Requirement for Foxd3 in the maintenance of neural crest progenitors.Development 135: 1615–1624.

PubMed Link

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