Materials and Methods

Quiz Content

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. For medical studies include (select all that apply):

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. Does the following example provide sufficient detail?
All samples were centrifuged.

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. Does the following example include unnecessary detail?
CD4+ CD44high T cell purification
We were interested in examining the gene expression of the anergic portion of T cells in Jak3 KO mice that are CD4+ CD44high. To obtain the CD4+ CD44high T cells used for RNA isolation, spleens were removed from Jak3 KO and Jak3 Het mice at 8 to 10 weeks of age. Splenocytes were isolated by homogenizing the tissue with frosted glass slides (Fisher Scientific, Pittsburgh, PA).

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. Does the following example include unnecessary information?
Cells were scraped out of the wells and resuspended in a 1.5 ml Eppendorf tube.

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. Which of the following are common problems of the Materials and Methods section? Select all that apply.

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. Select the better sentence.

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. Select the better sentence.

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