This is a technique we use for bloody samples.
We receive bloody effusion ---- and acetic samples,
we have to split the cells from all the blood.
So we use a blood separating technique called histopaque and it’s a density gradient separation,
we put a special fluid into the conical with the bloody fluid
and we spin it for half an hour and it gives us this result
where it splits the sample into layers and as you can see where the blood comes to the bottom
of the conical and there is a buffy layer just underneath the yellow layer which is all the cells.
We then have to sample those cells to put them on the slides to screen them.
To extract the layer cells in the middle, we have to place the pipette
down to the middle of the sample and try collect that buffy --- layer just underneath the yellow part.
--- circling all around to try to get as much as possible,
we then put hat buffy layer into a separate conical to which we then add another preservative.
Once we add the preservative we --- the sample again
and we get a ---- we have to remove the supernatant
down to where the --- is and we then add twelve drops
of more preservative and then once having done this we get a real quick, quick mix.
We then pipette three drops into each cytospin funnel.
And then it is at this point the sytospin funnel goes into the centrifuge again
and is spun for three minutes and the prep will be made and then stained for screening.